Welcome to Good Citizenship: RNA-Seq!

Welcome to Good citizenship made easy: A step-by-step guide to submitting your RNA-Seq reads and assemblies to NCBI.

RNA-Seq has facilitated an explosion of transcriptome studies in non-model organisms and contributed to our understanding of diverse aspects of evolutionary biology. Given that many RNA-Seq studies rely on public funding, we regard the open sharing raw data as an ethical imperative. In addition, most journals have data availability requirements that oblige researchers to publically share their data.

The databases housed at the National Center for Biotechnology Information (NCBI) are a popular choice for data deposition, but the submission of RNA-Seq data to NCBI can be challenging. We have often felt the need for a detailed, all-in-one-place, presentation of the steps required to submit RNA-Seq reads and assemblies to NCBI, so we decided to create one.

From the creation of an NCBI user account to the submission of an assembled transcriptome, this step-by-step guide will show you how it's done. We hope this contribution will allow scientists of any experience level to easily share the data they have worked so hard to generate, and make NCBI an even more valuable resource than it is today.