Creating a BioProject

4.        Go to https://submit.ncbi.nlm.nih.gov/subs/bioproject/ and sign in to NCBI with your user account.

Click on the BioProject link and choose New submission.

 

During this step, the submitter will provide information about the sequencing project, including organism information, funding sources, etc. The submission process progresses through seven fillable forms (presented as tabs at the top of the page). In order, these are:

 

        a.   Submitter: Provide information about the person submitting the data and the submitting organization (typically, this will be the submitter's organizational affiliation). An email address from the submitting organization's domain is required. If desired, again a shared submission group can be created.

 

        b.   Project type: Many Project data types are available; at this stage, the user should select Raw sequence reads. Also on this tab, the user must define the "sample scope", which will depend upon the biological entities included in the study. Monoisolate studies involve a single individual OR projects that result in a single transcriptome assembly, even if multiple individuals were pooled for sequencing; Multiisolate studies involve multiple individuals or strains from a single species; Multi-species studies involve individuals from 2+ different species; Environment studies involve environmental samples (water, soil, etc.) whose species content is unknown; Synthetic studies involve synthetically created samples; and Other is a catchall that allows the user to manually define the sample scope.

        c.   Target: Here information about the organism (or group of organisms) sequenced can be entered. The Organism name should be broad enough to cover all the included species (if multiple species within Genus are included, use Genus as the organism name).

 

        d.   General info: Here the user assigns a release date for the data, which can occur immediately upon submission or be delayed until publication (or until a specified future date). In addition, the user must choose a Project title and a Public description. These fields will be visible to the public once your submission is complete and released for public viewing. The user here defines the Relevance of the BioProject (Agricultural, Evolution, etc.). Additional (optional) fields are available on this tab: External Links, where the user can provide relevant web pages (such as a project page for the sequencing project or a personal research page describing the research goals); Select Your Grants, where the user can provide funding information; Consortium name, if the study is part of a consortium project; and, Data provider, if the data were not generated by the submitter.

 

        e.  BioSample: Here the user can provide a previously created BioSample ID. However, only single BioSamples can be registered this way. If the user's project involves multiple samples (i.e. if it was a Batch/Multiple BioSamples submission), the user should complete BioProject creation without including any BioSample information and then submit the BioSamples separately, including the BioProject ID in the submission. Click Continue without selecting a BioSample to skip this step.

 

        f.   Publications: If publications related to the BioProject already exist, they can be entered here. Future publications can be added at a later date.

 

        g.   Overview: Here the user can review all the submission parts and decide if it is ready to submit. Check carefully for any errors-once the submission is complete, changes can only be made by emailing the BioProject help desk! After the user is satisfied with all entries, hit submit.

 

Once the request is processed, you will receive a confirmation email containing your Submission Title, Submission ID, and BioProject ID (in the form PRJNAxxxxxx).

5.  Submit